Invitrogen midiprep manual

The PureYield™ Plasmid Midiprep System is designed to purify –µg of plasmid DNA with A /A from a 50–ml overnight culture of bacteria, transformed with a high-copy-number plasmid, with a total biomass (O.D. of culture × volume of culture) of – Larger amounts of biomass (e.g., up to ml ofFile Size: 1MB. Description. The PureLink Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from www.doorway.ru in about 30 minutes, using silica membrane columns. Features of the PureLink™ Quick Plasmid Miniprep Kit: Fast —ready-to-use plasmid DNA typically in about 30 min. Flexible —formats work with vacuum and Brand: InvitrogenTrade. Steps Midiprep Procedure Details 1 Equilibrate 1. Add 10 mL Equilibration Buffer (EQ1) to the HiPure Midi Column. Allow the solution to drain by gravity flow. 2 Harvest g for 10 min. Discard all medium. 3 Resuspend 3. Add 4 mL Resuspension Buffer (R3) with RNase A to the cell pellet and resuspend the pellet until it is homogeneous. 4 Lyse 4.

The PureLink® Quick Plasmid Miniprep Kit is designed for rapid isolation of sequencing-grade plasmid DNA from www.doorway.ru in minutes, using silica membrane columns. Features of the PureLink® Quick Plasmid Miniprep Kit: • Fast —ready-to-use plasmid DNA typically in minutes. • Flexible —formats work with vacuum and centrifugation. The PureLink® HiPure Plasmid Midiprep Kit is designed to isolate transfection-grade plasmid DNA from E. www.doorway.ru Midiprep Kit protocol will typically yield – µg plasmid DNA from 15–25 mL bacterial culture, at a purity that is comparable to that achieved by two passes through cesium chloride gradients. QIAGEN Plasmid Purification Handbook 08/ 7 EndoFree® Plasmid Kits EndoFree Plasmid Kit Maxi (10) Mega (5) Giga (5) Catalog no. QIAGEN-tip 10 – –.

Qiagen kit data derived from product instruction manuals. CsCl data derived from published literature. [Figure 2] PureLink™ HiPure Plasmid Filter Maxiprep. The PureLink™ HiPure Plasmid DNA Purification Kits use a patented anion-exchange resin to purify plasmid DNA to a level equivalent to two passes through CsCl. This manual is supplied with the following products. JetStar ™ Plasmid Midiprep Kit Genomed or Invitrogen that may be used with the JetStar.